AbstractGC - MS and UV - spectrophotometric investigation of accelerated lipid peroxidation and cell destruction in cultured human breast cancer cells (ZR-75-1) produced by gamma-linolenic acid and iron. Lipid peroxidation and its anticancer effect induced by gamma-linolenic and iron (GLA + Fe) were studied by ultraviolet-spectrometric (UV) and gas chromatographic-mass spectrometric (GC-MS) methods. When both were supplemented with GLA + Fe, there were far more dead cells in human breast cancer (ZR-75-1) cultures than in human normal skin fibroblasts (CCD 41-Sk). Accelerated lipid peroxidation was confirmed by the formation of conjugated dienes (CD) in the polyunsaturated fatty acids (PUFAS) and by the formation of hydroperoxyl group (OOH) indicated by the conversion of triphenylphosphine(TPP) to its oxide (TPPO) only in ZR-75-1 cells but not in CCD 41-Sk cells treated with GLA + Fe. Vitamin E inhibited the enhancement of peroxidation and cell killing produced by Fe in ZR-75-1 cells. It was shown that an accelerated production of lipid peroxides detected as CD and OOH-TPP reaction in cancer cells (ZR-75-1) was closely associated with the cancer cell killing produced by GLA + Fe. These findings suggest a lipid peroxidation mechanism specific to malignant cells to be investigated further, which might open the possibility of applying PUFA such as GLA as a highly selective antineoplastic agent and a sensitive reagent to detect malignancy.
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